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The reaction mixture consisted of 1.53 mL phosphate buffer (0.05 M, pH 7.0), 0.1 mL of ethylene diaminetetra acetic acid (1 mM), 0.1 mL of sodium azide (1 mM), 0.1 mL of glutathione (1 mM), 0.1 mL of NADPH (0.2 mM), 0.01 mL of hydrogen peroxide (0.25 mM) and 0.02 mL of PMS in a final volume of 3 mL

Quach HT, Johnson DB, LeBoeuf NR, Zwerner JP, Dewan AK

The ingredients in the 25% Vitamin C + Glutathione Clinical Serum are generally considered safe to use during pregnancy or while breastfeeding

We also thank Alexei Rohou, Axel Brilot, Tamir Gonen, and Meghna Gupta for helpful discussions on cryo-EM map refinement and model building, and members of the Tomasiak lab and Robert Stroud for helpful discussions and critical reading of this manuscript