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102 In vitro treatment with 1-h H 2 O 2 demonstrated that the catalytic activity of POLG was reduced to 50% of the original level with significantly lower DNA binding efficiency

After perfusion, liver tissue was carefully dissociated using sterile fine-tipped forceps, and the resulting cell suspension was filtered through a 100 m sterile strainer (Fisherbrand, 22-363-549) to remove debris and undigested fragments

This powerful antioxidant therapy helps protect brain cells from oxidative stress and supports cognitive function, providing relief from symptoms and enhancing quality of life

Glutathione-Related Enzymes and Proteins: A review