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Mice were randomly identified using telemetric sensors (Biolog-Animal, Paris) implanted subcutaneously in the dorsal region, under general anesthesia with 4% isoflurane (Vetflurane, Virbac, France)

The administration of candesartan is sufficient to block the isolation stress (24h isolation in individual metabolic cages)-induced increased binding of AT 1 R to PVN and to reduce the HPA response to stress (157)( Table 4 )

Common symptoms of even mild dehydration include decreased urination, cramping in muscles, headaches, confusion, and extreme thirst