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How does the combination of glutathione and Vitamin C enhance the efficacy of supplementation

Statistical analysis Computational survival analysis of OS and DFS based on GPX4 expression was completed using a log-rank test of the survival curves generated using the KaplanMeier method

Western blot analysis Cells were lysed in lysis buffer [50 mM Tris-HCl (pH 7.5), 1% NP-40, 0.5% (v/v) sodium deoxycholate, 150 mM NaCl and 1 Halt protease and phosphatase inhibitor mixture (Pierce)] on ice for 1 h

Step 2: Treatment Site Preparation The injection site is cleansed using medical-grade antiseptic protocols to maintain a sterile treatment environment