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Since mouse microbiome profiles often highly correlate with body weight and fat composition, this study was designed to test whether the cecal microbiomes of DIO mice treated with NNMTi and LD were comparable to the microbiomes of age-matched lean counterparts and distinct from microbiomes of DIO mice maintained on a high-fat Western diet (WD) or subjected to LD switch alone

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The pharmacokinetic parameters were calculated using the mean concentration and Watson LIMS software according to the non-atrioventricular model

Future investigations should test dose, timing, and ingredient patterns prospectively to clarify causal pathways and to inform targeted education, policy, and clinical practice