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The intestine as an endocrine organ and the role of gut hormones in metabolic regulation

Stomach acid and enzymes free vitamin B 12 from food, allowing it to bind to R-protein (formerly known as transcobalamin I and now called haptocorrin), found in saliva and gastric fluids

Proteins expression was induced by addition of 0.5 mM isopropyl--D-thiogalactopyranoside (IPTG) at 25 C for overnight

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