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However, mass spectrometry, while advantageous for analyzing whole cell association, has principal limitations as currently reported: (a) restricted throughput capacity and (b) would ambiguously define molecule location unless additional, careful fractionation methods are included to show periplasmic or cytoplasmic accumulation 18 , and (c) needs compounds to be mass active

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Fasting is required for rescuing hippocampal-dependent long-term memory in both sexes We examined the contribution of fasting on CR-induced improvements in cognition by performing behavioral assays on 12-month-old 3xTg and NTg mice that had been fed either an AL, DL, or CR diet prior to sacrificing them for the histological assays described above