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In the absence of transition metals, ascorbic acid can diminish the incidence of mutations caused by H 2 O 2 in human cells [165]

Acknowledgements This investigation was funded in part by the National Institutes of Health through grants R01 NS055095 (RAG) and in a facility constructed with support from Research Facilities Improvement Program Grant C06 RR15482 from the National Center for Research Resources, NIH

Rosen RB

Then, samples were first fixed with 50% of culture medium and 50% of 2% PFA with 2% of glutaraldehyde Hepes buffer 0.1 M at pH 7.4 for 1 h before being fixed with 2% of paraformaldehyde (PFA) with 2% of glutaraldehyde Hepes buffer 0.1 M for one night at 4 C