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Oxidative stress and antioxidant strategies in newborns

Afterwards, the sections were washed with Triton X (0.1%) three times (10 min each), followed by incubation with the secondary antibodies conjugated and diluted in the blocking buffer at room temperature for 2 h (see Supplementary Table 4 for secondary antibodies used in this study)

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This makes it vital to energy metabolism in all living organisms