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The measurement methods for all chemical compositions were in accordance with the description provided by the Chinese tobacco industry standard method ( 2.3 Metagenome sequencing and data analysis Microbial genome extraction, PCR amplification, library construction, sequencing, and routine data analysis were conducted by Biomarker Technologies ( Metagenome sequencing was performed on the Illumina Hiseq2500 platform following standard protocols utilizing the shotgun sequencing method