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To investigate the transcriptional regulatory effect of Gln on the GGCT gene, a 2000-bp sequence of the GGCT gene promoter region was inserted into the pGL4-Basic vector

The systems biology omics technologies (transcriptomics, proteomics and metabolomics) have been used to discover potential translational biomarkers of liver injury

Identification of the exact mechanism for the lethal effect of silencing the enzyme requires further experiments, as do the effects of inhibitors, which are not necessarily solely due to loss of enzyme activity, because the inhibited enzyme may also have a gain of function leading to cell death [72]

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