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Subcellular fractionation Nuclear, mitochondrial and cytosolic fractions were separated using the Mitochondria Isolation Kit for Cultured Cells (89874, ThermoFisher Scientific) according to the manufacturers specifications

The Tuesday before [I scaled the pole] we had a meeting

Even though hookah use in the western world is a recent trend, it has existed for a millennium, emerging in the North Western provinces of India, spreading to Iran, the Arab world, and Turkey and now gaining popularity in the USA and Europe [2]

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