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Commonly known as free radicals, ROS are primarily quenched by the two principal components of the antioxidant defence system of the lens: Glutathione (GSH) and ascorbic acid [10]

Most researchers agree that natural glutathione production begins to decline at a rate of about 1% to 2% per year starting in our early twenties

GSH/GSSG is a reliable indicator of cellular redox status, and a chronic reduction in GSH/GSSG reflects a reduced antioxidant capacity and increased vulnerability to oxidative damage

His 18 plays a critical role in catalysis by removing resonance stabilization in the carboxylate group and activating the oxyanion for attack