The results showed that after FOXO4-DRI treatment, the p-P53/P53 ratio significantly increased in the nucleus, cytoplasm, and total protein samples (Figure 5D)
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(100) has provided further mechanistic insights by employing UPLC-QE-HRMS, identifying 31 prototype compounds, 60 phase I metabolites, and 23 phase II metabolites of Scrophulariae Radix in vivo
Besides the formation of GSSG, the simulations revealed GSOH as oxidation product for GSH after H abstraction, and GSOH can be further oxidised to GSO 2 H and GSO 3 H, while the latter might also be produced via intermediate glutathione peroxide formation upon superoxide addition