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J Immunol (2010) 184:4119

All tissue samples were rapidly frozen and stored at 80 C until further processing [2]

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However, PAI-1 carbonylation has been demonstrated to influence its functional activity and structural stability [38], therefore carbonylated PAI-1 may exhibit reduced activity, and we hypothesize that it could trigger a compensatory mechanism leading to an increase in its overall concentration observed in our cohort