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Many methods are tedious and time consuming
cell culture supernatant or ascites and for Immunoprecipitation and Co-Immunoprecipitation of antigens from cell or tissue extracts
It contains two non-coded amino acid residues (Aib
which is involved in the maintenance of peripheral tolerance to self-antigens
Phorbol 12-myristate 13-acetate [PMA] Pack Size:25 mg Many methods are tedious andPMA TPA is the most commonly used phorbol ester. Binds to and activates protein kinase C (PKC) at nM concentrations. Induces cell growth arrest through a variety of pathways including the mitogen activated protein kinases (MAPKs), p38 and c Jun N terminal kinase (JNK) pathways mediated by cyclin dependent kinase (CDK) inhibitors such as p21WAF1 CIP1, p27KIP1, p15 and p16. Potent mouse skin tumor promoter. Promoter of inducible NOS (iNOS; NOS II).
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