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Backbone and insert fragment were ligated and transformed by heat shock into NEB 5 competent cells following the provided standard procedure of the vendor

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SAMe application alleviated these injuries in rats that underwent BDL ( Mat1a in mice resulted in depleted liver SAMe levels and reduced GSH levels, hypermethioninemia, marked changes in the expression of many enzymes of Met metabolism, and increased expression of many acute-phase markers and growth-related genes ( Mat1a - / - mice more susceptible to liver injury ( Mitochondria, as an energy factory in eukaryotic cells, are the primary source of reactive oxygen species (ROS) in hypoxic cells and participate in the regulation of redox homeostasis