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Each sample was resuspended in 20 l of LC/MS grade water and 10 l per sample was injected into a 5500 QTRAP, a hybrid triple quadrupole/linear ion trap mass spectrometer, using a quantitative polar metabolomics profiling platform with selected reaction monitoring (SRM) that covers all major metabolic pathways

GHKCu limits this by reducing pro-inflammatory cytokines (e.g., IL-6, TNF-) and promoting antioxidant defenses

150 mm x 2.1 mm 2.6 M 150 , Thermo Fisher Scientific) using a Vanquish Horizon UHPLC system (Thermo Fisher Scientific, Germering, Germany) coupled on-line to an Orbitrap Exploris 240 mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) equipped with a HESI source

Dynamic models