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Biochem Mosc

Indirect evidence to support this adenoma-carcinoma sequence includes the following: 1) prevalence rates cosegregate within populations, 2) subsite distribution patterns within the colorectum are similar, 3) benign adenomatous tissue is often juxtaposed with invasive cancer in early- stage malignancies, and 4) incidence rates of CRC are decreased by endoscopic polypectomy

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Organoids were embedded in 50 l of Matrigel (CLS354263, Corning, USA) and cultured with 500 l of modified medium (PRS-ICM-3D, PRECEDO, China) in 24-well plates