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Similar content being viewed by others Log in or create a free account to read this content Gain free access to this article, as well as selected content from this journal and more on nature.com or Abbreviations A : amyloid AD: Alzheimer's disease AMS: 4-acetamido-4-maleimidyl-stilbene-2,2-disulfonate ASK1: apoptosis signal-regulating kinase 1 BCA: bicinchoninic acid CERAD: Consortium to establish a Registry for Alzheimer's disease Daxx: death-associated protein DSM-IV: Diagnostic and Statistical Manual of Mental Disorders-IV DTT: dithiothreitol ERK: extracellular signal-regulated kinase GFAP: glial fibrillary acidic protein GRX1: glutaredoxin 1 GSH: reduced glutathione GSSG: oxidized glutathione HIV: human immunodeficiency virus IGF-I: insulin-like growth factor-I JNK: c-Jun N-terminal kinase MAPKKK: mitogen-activated protein kinase kinase kinase MCB: monochlorobimane MEM: minimum essential medium MTT: 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2 H -tetrazolium bromide ORF: open reading frame PI3K: phosphatidylinositol 3-kinase PLSD: post least significant distance PMSF: phenylmethylsulfonyl fluoride TCA: trichloroacetic acid TRX1: thioredoxin 1 TRXR: thioredoxin reductase References Hardy JA and Higgins GA (1992) Alzheimer's disease: the amyloid cascade hypothesis

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Step 5: Inject water into peptide vial Insert needle through the peptide vial's rubber stopper