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Procedures This randomized clinical trial took place between February 2019 and January 2020

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In vitro models have been employed to examine differential gene expression, revealing that e-cigarette aerosol, though less cytotoxic to human bronchial epithelial cells, induces distinct transcriptomic signatures with or without added nicotine 28

Its important to note that these effects can vary depending on the duration and intensity of smoking, as well as individual susceptibility