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GPX4 genetic silencing in vitro The lentiviral plasmids, either non-targeted vector (pLKO.1-shNT) or pLKO.1-shGPX4 plasmid DNAs (Sigma-Aldrich) were transfected along with packaging and envelop plasmids psPAX2 and pMD2.G into HEK293T cells

For example, choosing 2.5mg/mL concentration requires 2mL bacteriostatic water and delivers 250mcg per 0.1mL (10 units on a U-100 syringe)

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We first consider how the accumulation of membrane lipid peroxides leads to the execution of ferroptosis by altering ion transport across the plasma membrane