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After treatment, cells were immediately analyzed for apoptosis and the cell cycle using a BD FACSCelesta Cell Analyzer with an Annexin V-FITC/PI Apoptosis Detection Kit and PI/RNase Staining Buffer (BD Biosciences, Franklin Lakes, NJ, USA), respectively, according to the manufacturers instructions

Somatropin is FDA-approved for genuine medical indications such as growth-hormone deficiency, but use for anti-aging, bodybuilding or body-composition is off-label and not approved for those purposes

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NAD+ serves as an essential coenzyme for hundreds of enzymatic reactions, including those involved in energy production, DNA repair, and cellular signaling