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Step 3: For overnight repair, apply a thicker layer before bed

The microneedling creates micro-channels that deliver copper peptides directly to the dermal papilla the exact target cells identified in Pyo et al.'s 2007 work

For GSH assay, cells were treated in Earles balanced salt solution (EBSS), supplemented with 25 mM glucose, 4 mM glutamine, 1 X MEM vitamin solution (Life technologies, CA, USA), 0.22% wt/vol NaHCO 3 , 1 mM sodium pyruvate, 0.2% BSA, 25 mM HEPES, 100 U/ml penicillin and 100 g/ml streptomycin in the presence or absence of 1 X MEM amino acid solution (Gibco, CA, USA) with or without 100 M cystine

The release of mtRNA from mitochondria to cytoplasm also stimulates inflammation and immunity