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Some studies indicate that DSIP may influence endogenous opioid signaling, although its effects are not consistently reversed by opioid antagonists, suggesting indirect modulation rather than direct receptor agonism [9]

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Production and purification of recombinant proteins The constructs corresponding to human FOXO4 (residues 86 206) and human p53 (residues 1 312), were purchased from Genscript in a pUC cloning vector