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The toxicity increased with the increase in the sulfadiazine concentration extracted by HF-LPME at different pH values, suggesting that the HF-LPME extracted concentration can be used to estimate the toxicity of sulfadiazine independent of the ambient pH, that was the concentrations of sulfadiazine in the test solutions measured by HF-LPME and the corresponding immobilization-concentration-response equation were used to calculate the immobilization ratio

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