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In a study of male ICR mice exposed five days per week for two weeks to mainstream smoke from commercial, unfiltered, high-tar cigarettes (1-second puff of 20-mL volume at 10-second intervals and 45 puffs per cigarette), the lungs showed evidence of senescence of alveolar epithelial cells (increased -galactosidase activity, lipofuscin accumulation, and P21 CIP1/WAF1/SDI1 protein in type II cells) (Tsuji et al

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