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Euthanasia and tissue collection At the end of the experiment (24 h after detorsion), all animals were euthanized under deep anesthesia via an overdose of ketamine (50 mg/kg) and xylazine (10 mg/kg)

Funding Expenses related to the article publication are supported by the funding of the National Natural Science Foundation of China (82260036), Guizhou Provincial Science and Technology Projects (QKHPTRC-CXTD(2021)010), and the Collaborative Innovation Center of the Chinese Ministry of Education (JKJTH-2020-39)

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It influences gene expression related to detoxification, stress response, and apoptosis, supporting models investigating aging, longevity, and age-related disease prevention