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A flask containing 35 mL of LB media was then inoculated with the overnight culture at a ratio of 1:100 in the presence of 100 g/mL ampicillin and cells were grown at 37 C for 2 h, or until the optical density at 600 nm reached 0.2 when cultures were induced with 1 mM Isopropyl -d-1-thiogalactopyranoside (IPTG) at 37 C for 2 h in a shaker incubator

Additionally, the findings reveal that DSIP-CBBBP crosses the blood-brain barrier more effectively than traditional peptides, potentially enhancing its efficacy in vivo

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