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After 72 h treatments, 0.5 g ml 1 propidium iodide (Sigma-Aldrich, P4864) and 1.4 g ml 1 Hoechst 33342 (Invitrogen, H3570) were added to cells

If your research rationale genuinely requires high-dose exploration, calculate injection volumes using maximum practical concentration (10mg/ml) and consider dividing doses across multiple injection sites if individual volumes exceed 0.3ml to avoid tissue irritation and ensure complete absorption

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