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TMV RNA Detection by Real-time RT-PCR From mouse lungs, TMV RNA was detected and quantified using two systems of real-time reverse transcription (RT)-PCR, one targeting a region that corresponds to a fragment of the capsid gene and the other targeting the replicase gene (Table 1), with the SuperScript III Platinum One-Step Quantitative RT-PCR System (Invitrogen Life Technologies, Carlsbad, Calif., USA) on a Mx3000P thermocycler (Stratagene, La Jolla, CA 92037 USA) under the following conditions: 50C for 30 min for RT, 95C for 2 min for initial denaturation and 45 cycles including 95C for 30 sec and 60C for 1 minute

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