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The brain of each mouse was then harvested, and 20 mg of tumor tissue was dissected for homogenization in 0.2 ml of lysis buffer, which is prepared by mixing two volumes of PBS with one volume of Stop buffer provided by the glucose uptake assay kit (Promega #J1342)

Fistula Healing as Support BPC 157 application successfully cured various fistulas (Sikiric et al., 2020a)

The Lipofectamine 3000 Kit (L3000015, Thermo Fisher Scientific) was used to transfect the cells with two plasmids: the 2.5 g negative control plasmid pcDNA3.1 and the 2.5 g pcDNA3.1 MPO plasmid