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For CytB, the primers CytB 876R and CytB 424F (Boore and Brown, 2000) were used with the following cycling parameters: 2 min at 95 C, (15 s at 95 C, 15 s at 47 C, 45 s at 67 C) x 40 cycles, 5 min at 68 C, and finally cooling at 10 C

This type of artifact is audible with most portable Bluetooth speakers, but given the size of the Rockster Go 2s internal speakers and bass radiator, we expected a little less limiting

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Cigarette smuggling is especially prevalent when there is a significant different in tax rates between two bordering states with population centers living near the border