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For cell samples, cells were lysed with lysis buffer (50 mM Tris-HCl [pH 8.0], 1% Triton X-100, 0.5% Nonidet P-40, 10 mM dithiothreitol, 1% protease inhibitor cocktail, 150 mM NaCl, and 5 mM EDTA) on ice for 30 min, followed by centrifugation (12,000 g , 20 min, and 4 C)

The utilization of plasma-activated solutions (PAS), like water, saline, Ringers lactate, culture medium, and phosphate-buffered saline (PBS), as an important complementary application way to direct plasma treatment, has demonstrated favorable treatment effects and application prospects in sterilization, tumor treatment and skin disease management 23,24,25

Annu Rev Entomol

Bernstein, L